| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.250729 |
| Chromosome: | chromosome 7 |
| Location: | 2899281 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g332550 | PSL1 | Signal peptide peptidase, eukaryotic-type; (1 of 1) PTHR12174//PTHR12174:SF23 - SIGNAL PEPTIDE PEPTIDASE // MINOR HISTOCOMPATIBILITY ANTIGEN H13 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGCTGTTTCCACGGGCGGGCAGCGCGGA |
| Internal bar code: | CTGTCTTACCGTATAGTCGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 741 |
| LEAP-Seq percent confirming: | 99.4667 |
| LEAP-Seq n confirming: | 3730 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACCTTGGATTGCTTTTGG |
| Suggested primer 2: | TTCGGTCTGTTCCTGCTCTT |