| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.250752 |
| Chromosome: | chromosome 16 |
| Location: | 6533567 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g672650 | MITC14,MCP14 | Mitochondrial substrate carrier protein; (1 of 1) K15104 - solute carrier family 25 (mitochondrial oxoglutarate transporter), member 11 (SLC25A11, OGC) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTGTTTGATGATTACTTCCAGGTCGGCGA |
| Internal bar code: | TCGGAACCCTCAAGAGCCGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 531 |
| LEAP-Seq percent confirming: | 73.0769 |
| LEAP-Seq n confirming: | 38 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGACAAAAATTTCCGCTCC |
| Suggested primer 2: | GTACGAGGATGTGGGCAAGT |