Insertion junction: LMJ.RY0402.250780_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.250780
Chromosome:chromosome 16
Location:1116438
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre16.g649833 (1 of 1) IPR001841//IPR003903 - Zinc finger, RING-type // Ubiquitin interacting motif 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCCTGTGTGTCCGCGGTTGCGGCAAGACT
Internal bar code:CACTACCCCCTCTCGAGTCGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:398
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:AACGAAAAGGTTTTGTTGCG
Suggested primer 2:GCAAGCACGATAACGAGGAT