| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.250782 |
| Chromosome: | chromosome 7 |
| Location: | 3445444 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g335400 | EttA | Putative soluble chloroplast ABCF protein, relatated to bacterial EttA which throttles translation speed; (1 of 1) PTHR19211:SF7 - ABC TRANSPORTER-RELATED PROTEIN | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTGAGCCGGCCCTGGCGGCGGTGAAGGGC |
| Internal bar code: | AGGGAAGTCGTGTCTGGAGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 239 |
| LEAP-Seq percent confirming: | 98.1366 |
| LEAP-Seq n confirming: | 158 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTAGGGAAGAGGATGGAGG |
| Suggested primer 2: | TAGTGGTGGCCGTGTGTAAA |