Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.250791 |
Chromosome: | chromosome_2 |
Location: | 84625 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre02.g073750 | KIN9-2,KLP1 | Kinesin motor protein, kinesin-9 (Kif9) family | sense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TCAACCGGCGCAGGCCCCGCTGCATCACCG |
Internal bar code: | GGCTCGGGCTTGTCCGCCGTAC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1039 |
LEAP-Seq percent confirming: | 99.8107 |
LEAP-Seq n confirming: | 10544 |
LEAP-Seq n nonconfirming: | 20 |
LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AGAATGATTGAAACAGCCCG |
Suggested primer 2: | CCTCACCCTAGCTTGTCAGC |