| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.250810 |
| Chromosome: | chromosome 3 |
| Location: | 6637812 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g196900 | (1 of 1) PTHR10809:SF6 - AT11025P-RELATED | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCCAGTCTGAGATTCAAACATTTAGAAG |
| Internal bar code: | TCGTAATGGTAGTAGCCTGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 693 |
| LEAP-Seq percent confirming: | 96.7742 |
| LEAP-Seq n confirming: | 240 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCCTTTGAGCTGCTGGTTC |
| Suggested primer 2: | GGTGCCTATCTAGCAGTCGC |