Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.250838 |
Chromosome: | chromosome_7 |
Location: | 631786 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre07.g316992 | sense | intron|outside_mRNA |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TTGCACACACACACACACCACACACATACA |
Internal bar code: | CACGCGGCCTCGAGATGATTTG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 298 |
LEAP-Seq percent confirming: | 100.0 |
LEAP-Seq n confirming: | 1 |
LEAP-Seq n nonconfirming: | 0 |
LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CGCACACGCTCTTGAATCTA |
Suggested primer 2: | CGGTTTAACTTGGGCTGGTA |