| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.250857 |
| Chromosome: | chromosome 2 |
| Location: | 5079924 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g104850 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTTCCCAAACGGCGCGCGCACGACTATGC |
| Internal bar code: | CCATCACAATCACGGCAGCAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 530 |
| LEAP-Seq percent confirming: | 99.0731 |
| LEAP-Seq n confirming: | 962 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGATCCCCGTGTGTCAGTTA |
| Suggested primer 2: | ACCTAACCGTCACTCCCCTT |