| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.250874 |
| Chromosome: | chromosome 2 |
| Location: | 6749759 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g118450 | DNJ28 | (1 of 1) IPR000104//IPR001623//IPR012336 - Antifreeze protein, type I // DnaJ domain // Thioredoxin-like fold; DnaJ-like protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGGGTAGCTGACCTTGGCGGCGGCCGCGG |
| Internal bar code: | GGTCGGTAGGGCTTCGCACCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 327 |
| LEAP-Seq percent confirming: | 99.7015 |
| LEAP-Seq n confirming: | 334 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCAACTTATCCAGATCCGA |
| Suggested primer 2: | CCAAACATGCCTGATCCTCT |