Insertion junction: LMJ.RY0402.250885_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.250885
Chromosome:chromosome 7
Location:4386408
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre07.g341100 3'UTR|CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTCCCGGAGAGCGTTTGACACACATGCGC
Internal bar code:GACTCGCAGGTACATAGGGCCG

Confirmation - LEAP-Seq

LEAP-Seq distance:323
LEAP-Seq percent confirming:62.5
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:ATGTGCGCAGCTAAGAAGGT
Suggested primer 2:GACAGGCATGTCAGGGTTTT