| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.250912 |
| Chromosome: | chromosome 12 |
| Location: | 1561002 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g486800 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGGACGGACGCAGCTTGGATGAAAGCAAG |
| Internal bar code: | ACAGTGCACACGCCTGGTCCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 556 |
| LEAP-Seq percent confirming: | 95.0521 |
| LEAP-Seq n confirming: | 365 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCAGACCAGGGAACTGTTG |
| Suggested primer 2: | GTGACAGAGCTCCGTGAACA |