Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.250913 |
Chromosome: | chromosome_14 |
Location: | 2456222 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre14.g624950 | DHC10,IDA2,DYH1B | Flagellar inner arm dynein 1 heavy chain beta | sense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GTTCGACAAGGACAAGCTGGACGACTCGCT |
Internal bar code: | GGCAGCCAAGTCCAGAGCGCGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 416 |
LEAP-Seq percent confirming: | 100.0 |
LEAP-Seq n confirming: | 56 |
LEAP-Seq n nonconfirming: | 0 |
LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ACTACCGTGGACTCACCGAC |
Suggested primer 2: | TGATTGCGTGTCCTCAGAAG |