| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.250956 |
| Chromosome: | chromosome 7 |
| Location: | 99256 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g312817 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGCGAGTGTGGTACCCAGTTTGCGCGGT |
| Internal bar code: | GGTTGCGACCGTCGCACCGCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 541 |
| LEAP-Seq percent confirming: | 99.6084 |
| LEAP-Seq n confirming: | 1526 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAACGAATCTAATTGCCGC |
| Suggested primer 2: | ACATAGCCAAAACAGGTGCC |