| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.250972 |
| Chromosome: | chromosome 5 |
| Location: | 1482768 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g243801 | FAP387 | Flagellar Associated Protein 387 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTCCTCCTCCTCCTCTTCCTCACCCCCCC |
| Internal bar code: | GGCCATGGTCGCTGAGTATTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 233 |
| LEAP-Seq percent confirming: | 83.3935 |
| LEAP-Seq n confirming: | 231 |
| LEAP-Seq n nonconfirming: | 46 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGATATCGCACACGGTACAC |
| Suggested primer 2: | TAAGTAACTCCGGCGTCGTC |