| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.250972 |
| Chromosome: | chromosome_5 |
| Location: | 1482813 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre05.g243801 | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTGTTTGGAAGAGCATGGGGGAGGATGGGC |
| Internal bar code: | CAATTCTGTGCTATTACTAATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 214 |
| LEAP-Seq percent confirming: | 99.531 |
| LEAP-Seq n confirming: | 1061 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGATATCGCACACGGTACAC |
| Suggested primer 2: | TAAGTAACTCCGGCGTCGTC |