Insertion junction: LMJ.RY0402.251034_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.251034
Chromosome:chromosome 5
Location:1247103
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre05.g245352 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCATCCATACTTGACCCCATTGGAACCAA
Internal bar code:TCTTTGTGTTGGTTCTTGCGT

Confirmation - LEAP-Seq

LEAP-Seq distance:674
LEAP-Seq percent confirming:98.9583
LEAP-Seq n confirming:950
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:AAACTGTCAATGCTCCCGAC
Suggested primer 2:GGATTTAACGGACGCAGTGT