Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.251034 |
Chromosome: | chromosome 5 |
Location: | 1247103 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre05.g245352 | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCATCCATACTTGACCCCATTGGAACCAA |
Internal bar code: | TCTTTGTGTTGGTTCTTGCGT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 674 |
LEAP-Seq percent confirming: | 98.9583 |
LEAP-Seq n confirming: | 950 |
LEAP-Seq n nonconfirming: | 10 |
LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AAACTGTCAATGCTCCCGAC |
Suggested primer 2: | GGATTTAACGGACGCAGTGT |