| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.251039 |
| Chromosome: | chromosome 1 |
| Location: | 3624897 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g023350 | THO2 | Oligoendopeptidase; (1 of 1) 3.4.24.16 - Neurolysin / Neurotensin endopeptidase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGAGAAGGAATGCGGCAGGCGGAACAGGG |
| Internal bar code: | TTCAGACTCGTTCGATCATCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 25 |
| LEAP-Seq percent confirming: | 98.69 |
| LEAP-Seq n confirming: | 226 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCTGTTTCCCAAGATGGT |
| Suggested primer 2: | ATGCTTAAACCGTGCAAACC |