Insertion junction: LMJ.RY0402.251063_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.251063
Chromosome:chromosome 9
Location:6946416
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre09.g410500 PAP11 (1 of 1) IPR002934//IPR023214 - Polymerase, nucleotidyl transferase domain // HAD-like domain; Class-II RNA nucleotidyl transferase 11 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGGACCAGCAGGGGCGGAGGCGGCGCGGCG
Internal bar code:GTAGTCTGCATGGAGTTGGTGA

Confirmation - LEAP-Seq

LEAP-Seq distance:273
LEAP-Seq percent confirming:96.8825
LEAP-Seq n confirming:404
LEAP-Seq n nonconfirming:13
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:CACAATACACGCACAGGTCC
Suggested primer 2:CTTACTTGGCTTGACGCACA