| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.251089 |
| Chromosome: | chromosome 3 |
| Location: | 3089794 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g164650 | MOT56,MOT1 | Putative methyltransferase in motile organisms; (1 of 3) K05302 - SET domain-containing protein 6 (SETD6) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCGGGGCCGGGGGCCAAGGTCCCGGGCG |
| Internal bar code: | AAGTGTGTTATGCGTATACAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 0 |
| LEAP-Seq percent confirming: | 99.6016 |
| LEAP-Seq n confirming: | 250 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAATACCCACACCCACAACC |
| Suggested primer 2: | ATCGCCATACTCCCTCACTG |