| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.251137 |
| Chromosome: | chromosome 12 |
| Location: | 7959259 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g552200 | PHOB,PHO3,STA4,PHOB1 | (1 of 2) 2.4.1.1 - Glycogen phosphorylase / Polyphosphorylase; Starch phosphorylase PhoB | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCCCGACATGGGCGACCTGCTCAAGCTGG |
| Internal bar code: | CACTGGTTTGCGTAGTGACTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 359 |
| LEAP-Seq percent confirming: | 98.0263 |
| LEAP-Seq n confirming: | 149 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACATAGCAGGTGTCCCCAG |
| Suggested primer 2: | AGGAGAAACTGCAGACGCAT |