| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.251139 |
| Chromosome: | chromosome 2 |
| Location: | 4175795 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g098400 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCACTCGTAAAGGTCACGTGCTCCACTGGT |
| Internal bar code: | GTCGCATGGCTTTATGTTTAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 428 |
| LEAP-Seq percent confirming: | 99.8065 |
| LEAP-Seq n confirming: | 7736 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTGCCGTTCATCTATGGT |
| Suggested primer 2: | CTAATGACTTCGCTCCTCCG |