| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.251160 |
| Chromosome: | chromosome 5 |
| Location: | 392736 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g232950 | (1 of 1) K13110 - microfibrillar-associated protein 1 (MFAP1) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGAAATTGGTCTTCCTTATGCAGTTGGTA |
| Internal bar code: | AGGGGGGATGCAGTTTTTATAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 558 |
| LEAP-Seq percent confirming: | 99.081 |
| LEAP-Seq n confirming: | 3558 |
| LEAP-Seq n nonconfirming: | 33 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCCCTCTACCTAAAGTGGG |
| Suggested primer 2: | TTACCCCACATCTTCTTGCC |