| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.251162 |
| Chromosome: | chromosome 12 |
| Location: | 7910194 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g552650 | PAP8 | (1 of 1) PTHR23092:SF19 - NUCLEOTIDYLTRANSFERASE; Class-II RNA nucleotidyl transferase 8 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCACATACCATACCACACTGTGCTCGTAT |
| Internal bar code: | AGGCAGATTTGCCTCGGGGCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 554 |
| LEAP-Seq percent confirming: | 99.5138 |
| LEAP-Seq n confirming: | 1228 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTGTGTGATCCCGAGTATG |
| Suggested primer 2: | TCATGACGAACCTTGACAGC |