| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.251182 |
| Chromosome: | chromosome 6 |
| Location: | 6542171 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g293362 | (1 of 726) IPR011009 - Protein kinase-like domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGGGCCAATTGGTACTGGCGCTGGCCCTG |
| Internal bar code: | GTTAAGACCCCGGCGAACGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 678 |
| LEAP-Seq percent confirming: | 95.2752 |
| LEAP-Seq n confirming: | 6594 |
| LEAP-Seq n nonconfirming: | 327 |
| LEAP-Seq n unique pos: | 78 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCTTTGGTACATGGCGAAG |
| Suggested primer 2: | CGGGTGTCGTTGTAAGGAGT |