Insertion junction: LMJ.RY0402.251196_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.251196
Chromosome:chromosome 1
Location:3392843
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre01.g021550 (1 of 4) PTHR12298//PTHR12298:SF7 - PCDC2 PROGRAMMED CELL DEATH PROTEIN 2 -RELATED // SUBFAMILY NOT NAMED 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAACACACAGTAACCCGACCCGGTTGGGTG
Internal bar code:GTGTCCCATGAGCACACCAGAC

Confirmation - LEAP-Seq

LEAP-Seq distance:1036
LEAP-Seq percent confirming:99.2133
LEAP-Seq n confirming:5423
LEAP-Seq n nonconfirming:43
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:GAGCTGGGAGTCAAGGTCTG
Suggested primer 2:TGAGTCAGTGACAGGCAAGG