| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.251217 |
| Chromosome: | scaffold 18 |
| Location: | 45633 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre18.g748497 | (1 of 3) 3.1.3.66 - Phosphatidylinositol-3,4-bisphosphate 4-phosphatase / Phosphoinositide 4-phosphatase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATTCCGGATTGACCGAAGCGAGACGCCGC |
| Internal bar code: | CTTCATCACGCAAGCCGTCACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 540 |
| LEAP-Seq percent confirming: | 65.1355 |
| LEAP-Seq n confirming: | 865 |
| LEAP-Seq n nonconfirming: | 463 |
| LEAP-Seq n unique pos: | 59 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAGCATGTGTTGAAGAGCA |
| Suggested primer 2: | GGGAAGACTCAATCCCACAA |