| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.251221 |
| Chromosome: | chromosome 1 |
| Location: | 5644288 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g040200 | WNK2 | (1 of 5) K08867 - WNK lysine deficient protein kinase [EC:2.7.11.1] (WNK, PRKWNK); WNK protein kinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGACTGGAGTTCCAAGAGTTGCTTTTAC |
| Internal bar code: | GCGGCTGCGCTGTGGGGTAATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 743 |
| LEAP-Seq percent confirming: | 99.3473 |
| LEAP-Seq n confirming: | 2892 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGACGAGTCGTGCAGTATC |
| Suggested primer 2: | TTCGTGGGTAGGGCAATAAG |