| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.251260 |
| Chromosome: | chromosome 7 |
| Location: | 260500 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g313950 | (1 of 14) PF04577 - Protein of unknown function (DUF563) (DUF563) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGTCATGAATGTAACTCCCCCCCCCGCCC |
| Internal bar code: | AGAGCAAACACGGAAAGAGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 546 |
| LEAP-Seq percent confirming: | 86.3158 |
| LEAP-Seq n confirming: | 82 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCTCTCCTCCTCGCTCTTT |
| Suggested primer 2: | AGGGGGAATTTGGATACTGG |