| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.251280 |
| Chromosome: | chromosome 4 |
| Location: | 1793312 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g212050 | (1 of 2) IPR000104//IPR000253//IPR008984 - Antifreeze protein, type I // Forkhead-associated (FHA) domain // SMAD/FHA domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCTGGTTAAGTCGCTGACATAGAACTGAT |
| Internal bar code: | TCACAGTACTACCATAATTCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 698 |
| LEAP-Seq percent confirming: | 99.4455 |
| LEAP-Seq n confirming: | 538 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCTCACTCGACCACGTCTT |
| Suggested primer 2: | GCCCAGAGTTTGTCATGGAT |