| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.251305 |
| Chromosome: | chromosome 2 |
| Location: | 3439303 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g095114 | (1 of 228) IPR029063 - S-adenosyl-L-methionine-dependent methyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCACACAGCCGACCGGTCCGCCATGCAAGG |
| Internal bar code: | ATTCGGTGTGCCAGGCCTCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 605 |
| LEAP-Seq percent confirming: | 93.9951 |
| LEAP-Seq n confirming: | 767 |
| LEAP-Seq n nonconfirming: | 49 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCATGGGCTCAAAACAAT |
| Suggested primer 2: | CGAGTGGCGGTAGGTTGTAT |