| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.251308 |
| Chromosome: | chromosome 12 |
| Location: | 8845032 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g544900 | (1 of 1) PTHR16470:SF0 - UBIQUITIN DOMAIN-CONTAINING PROTEIN UBFD1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGAGAAGGGTGGGGTAGTTCATTCTAGAA |
| Internal bar code: | CGTTTACCCTGGTGGGGAAGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1214 |
| LEAP-Seq percent confirming: | 97.6812 |
| LEAP-Seq n confirming: | 337 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCTGATTGAAGGGAATGAA |
| Suggested primer 2: | ACGTGCTGAGCATGACAAAG |