| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.251310 |
| Chromosome: | chromosome_1 |
| Location: | 4028288 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g026200 | PPR9 | Pentatrichopeptide repeat protein | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GAGATGAGCATGCACTCTGGTTGGGCGGCG |
| Internal bar code: | GCGCCGCTGGATTGTGACCGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 499 |
| LEAP-Seq percent confirming: | 60.3407 |
| LEAP-Seq n confirming: | 4747 |
| LEAP-Seq n nonconfirming: | 3120 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGTACATATGCACAAACGC |
| Suggested primer 2: | ACTGTCATGACTCGACGCAC |