| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.251326 |
| Chromosome: | chromosome 1 |
| Location: | 2299834 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g012600 | GPM2,PGM2 | Phosphoglucomutase 2; (1 of 1) 5.4.2.7 - Phosphopentomutase / Phosphodeoxyribomutase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCAAATCTGTCCAACACACCAACGCACCG |
| Internal bar code: | GCCAGGGGGAGCCGCCCCAACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 329 |
| LEAP-Seq percent confirming: | 96.25 |
| LEAP-Seq n confirming: | 154 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCATGTGCTGGCTCTGGTA |
| Suggested primer 2: | GTGTATTTTTGCCAACCGCT |