| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.251353 |
| Chromosome: | chromosome 13 |
| Location: | 3735294 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g589400 | PRL6 | (1 of 14) PTHR10334 - CYSTEINE-RICH SECRETORY PROTEIN-RELATED; Predicted extracellular protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCCCGGCTTGCGGTGTGCCTGCTTGGGTT |
| Internal bar code: | GTCGTCGACCGGGATCTAGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 328 |
| LEAP-Seq percent confirming: | 99.7347 |
| LEAP-Seq n confirming: | 376 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGAAACACAGGTTGGCCTC |
| Suggested primer 2: | GGAAACGGAAGACTGCTGAG |