| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.251368 |
| Chromosome: | chromosome 12 |
| Location: | 8524081 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| intergenic |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGGTAGGCCCGAAAGGACCCAACACCGGC |
| Internal bar code: | CAGGATGGCGTTCGGCCCTGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 526 |
| LEAP-Seq percent confirming: | 97.4843 |
| LEAP-Seq n confirming: | 155 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCTCTCTTCATGCCAGAAC |
| Suggested primer 2: | GATAAGAGACGCGGACTTCG |