| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.251409 |
| Chromosome: | chromosome 3 |
| Location: | 3566082 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g168150 | (1 of 2) PF00069//PF12796 - Protein kinase domain (Pkinase) // Ankyrin repeats (3 copies) (Ank_2) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTTTCATCCTCCACTCCACACCCAGCGCT |
| Internal bar code: | GAATATGCTTACTCCACGCGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 773 |
| LEAP-Seq percent confirming: | 99.3932 |
| LEAP-Seq n confirming: | 3440 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACATTACGCTGGGGTCTGT |
| Suggested primer 2: | CCTGACATGCCAAACAAGTG |