| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.251414 |
| Chromosome: | chromosome 10 |
| Location: | 31051 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g417650 | (1 of 1) 2.1.1.207 - tRNA (cytidine(34)-2'-O)-methyltransferase / tRNA methyltransferase L | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCCTTGGCGCCAATTGGTCTCCCGTTGTC |
| Internal bar code: | CCATTACCCCCTCCAGGTTGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 299 |
| LEAP-Seq percent confirming: | 99.6787 |
| LEAP-Seq n confirming: | 3723 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTACGCCTTCCTCTTTGTCG |
| Suggested primer 2: | AACATCATCTGAAATCCGCC |