| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.251436 |
| Chromosome: | chromosome 3 |
| Location: | 7318493 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g206800 | HXT1 | (1 of 1) PTHR23500//PTHR23500:SF118 - FAMILY NOT NAMED // PLASTIDIC GLUCOSE TRANSPORTER 4; Plastidic glucose transporter | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGAGGCCGAGCTGCGCGCAGGAGCCAAGG |
| Internal bar code: | AAAGACTAGGCAGCGGCTCAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 886 |
| LEAP-Seq percent confirming: | 98.7475 |
| LEAP-Seq n confirming: | 14822 |
| LEAP-Seq n nonconfirming: | 188 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGACATGATGTTTGCCTGG |
| Suggested primer 2: | CATCGCCTCTATCTGCTTCC |