Insertion junction: LMJ.RY0402.251438_4

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.251438
Chromosome:chromosome 12
Location:4808067
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre12.g524450 PTP3 Protein tyrosine phosphatase; (1 of 1) PF00102//PF00782 - Protein-tyrosine phosphatase (Y_phosphatase) // Dual specificity phosphatase, catalytic domain (DSPc) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCTTGGCACAGTGCTCGCCCTGTCGGGAC
Internal bar code:AGTTGAACTAGCGTCTCATTCT

Confirmation - LEAP-Seq

LEAP-Seq distance:471
LEAP-Seq percent confirming:99.3322
LEAP-Seq n confirming:595
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CCCTTCAGTAATGCCACGTT
Suggested primer 2:TTTGTACGGAACCGCCTAAC