| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.251456 |
| Chromosome: | chromosome 7 |
| Location: | 5272873 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g349167 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATAATGGTTGTTCCGTTATTGGCAGGAAGC |
| Internal bar code: | ATTATATTTTGCGCGCCTAGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1068 |
| LEAP-Seq percent confirming: | 98.6301 |
| LEAP-Seq n confirming: | 26424 |
| LEAP-Seq n nonconfirming: | 367 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGTGATGAGGACAAGGAGA |
| Suggested primer 2: | TCTTGCGCCGAAGTAAGTTT |