| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.251497 |
| Chromosome: | chromosome 3 |
| Location: | 5222127 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g182800 | AGT2 | Alanine-glyoxylate transaminase; (1 of 1) K00827 - alanine-glyoxylate transaminase / (R)-3-amino-2-methylpropionate-pyruvate transaminase (AGXT2) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCTGCAAGAGTATCCCCATCACGCGAAAG |
| Internal bar code: | GAGGTTGAGTCCCTGTCCGAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 755 |
| LEAP-Seq percent confirming: | 99.0982 |
| LEAP-Seq n confirming: | 2967 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACTGCAGACTCCAAGGTCA |
| Suggested primer 2: | GCATCCCTCACATCCCTAGA |