| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.251498 |
| Chromosome: | chromosome 10 |
| Location: | 280858 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g419750 | RPB3 | DNA-directed RNA polymerase II, 36 kDa polypeptide; (1 of 1) K03011 - DNA-directed RNA polymerase II subunit RPB3 (RPB3, POLR2C) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGACGGATGTCGTGCTGCAAGGTTGCTGC |
| Internal bar code: | GTCACGCTATGGTGCGGCTAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 540 |
| LEAP-Seq percent confirming: | 98.2047 |
| LEAP-Seq n confirming: | 1094 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTGTGTGGACCTGGAGACC |
| Suggested primer 2: | AGTATGTCGTTACCGCCCAG |