| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.251515 |
| Chromosome: | chromosome 1 |
| Location: | 1042163 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g006050 | LF3 | Long Flagella protein 3; (1 of 781) IPR000104 - Antifreeze protein, type I | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATTGCGATCCGTGCTCAAGGTAACTGCTT |
| Internal bar code: | TTCGAATCGACCCATGGGATGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 577 |
| LEAP-Seq percent confirming: | 95.1807 |
| LEAP-Seq n confirming: | 79 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTGCCACCAGCCTATGTTT |
| Suggested primer 2: | GGCCTATTAGGGTAGCAGGG |