| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.251524 |
| Chromosome: | chromosome 16 |
| Location: | 1202541 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g650750 | FAP268 | (1 of 18) PTHR10891 - EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN; Flagellar Associated Protein 268 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGCTCCTAAGTGATATGCCGCAGCCGCAC |
| Internal bar code: | GGTCCGGAGCCTAGCCCCTCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 590 |
| LEAP-Seq percent confirming: | 98.929 |
| LEAP-Seq n confirming: | 1755 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 45 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCCAGGAGGAGGATGACAC |
| Suggested primer 2: | ATCGGGTGAATGGATGATGT |