| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.251540 |
| Chromosome: | chromosome 7 |
| Location: | 5476269 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g350850 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCCCCCGTACCCTTTTGCAGTTGTTGGTG |
| Internal bar code: | CAAGGCCGGCAGCAGCTGTGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 327 |
| LEAP-Seq percent confirming: | 98.9035 |
| LEAP-Seq n confirming: | 451 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTGACGCGGGCTATATTT |
| Suggested primer 2: | CCTGAGCTTTCTATTCCCCC |