Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.251544 |
Chromosome: | chromosome_1 |
Location: | 1015798 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre01.g005813 | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TCTACACCAAACGTCAGGACCGCAAGCGAA |
Internal bar code: | TGTGGTTCAACTTATTGATGTC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1076 |
LEAP-Seq percent confirming: | 99.7091 |
LEAP-Seq n confirming: | 8225 |
LEAP-Seq n nonconfirming: | 24 |
LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TAGCTTGGCTGTCGCCTTAT |
Suggested primer 2: | AGTCGGAGCCCATGTAACAC |