| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.251544 |
| Chromosome: | chromosome_13 |
| Location: | 4474998 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre13.g603600 | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ACAGACCATGCCGTACTGTAGAGCTGCACA |
| Internal bar code: | TCGTGACTCTGGGTGAAACGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 478 |
| LEAP-Seq percent confirming: | 92.4242 |
| LEAP-Seq n confirming: | 61 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGATTTACACGTCCCTGAT |
| Suggested primer 2: | TTGAAGAACAGCACGAATGC |