| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.251544 |
| Chromosome: | chromosome_13 |
| Location: | 4475004 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre13.g603600 | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGGGTGCAGTGCATGCACGTCCCTGCAGAT |
| Internal bar code: | TAATTATGACCCGGGCCCGGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 632 |
| LEAP-Seq percent confirming: | 90.0621 |
| LEAP-Seq n confirming: | 145 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGAAGAACAGCACGAATGC |
| Suggested primer 2: | GCGATTTACACGTCCCTGAT |