| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.251545 |
| Chromosome: | chromosome 10 |
| Location: | 4945904 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g455050 | (1 of 1) IPR000104//IPR003347//IPR006917 - Antifreeze protein, type I // JmjC domain // SOUL haem-binding protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACGCAGCACTTCCGTCACCAGCGGCTTCT |
| Internal bar code: | AACTCATTTCTTGCATTGATAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1 |
| LEAP-Seq percent confirming: | 64.7059 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGAGCAGAAAGGGACTTGC |
| Suggested primer 2: | TTCCCACAATGATGATGGAC |