| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.251549 |
| Chromosome: | chromosome 3 |
| Location: | 5126086 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| intergenic |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACTAGACACCACGTGGCCACAGGCAGAGA |
| Internal bar code: | ATAAACAGCGTCTGGCAAGGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 497 |
| LEAP-Seq percent confirming: | 97.8639 |
| LEAP-Seq n confirming: | 1970 |
| LEAP-Seq n nonconfirming: | 43 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCATCTTGTTGCGCTGTA |
| Suggested primer 2: | CAAGGCACTTCTGAAGGAGG |